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A single enamel or dentin specimen, presenting 6 mm in diameter and 1 mm high, was obtained from the labial surface of each crown using a trephine mill ( Moraes, São José dos Campos, SP, Brazil) and polishing paper (Extec, Essington, PA, USA).
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from the labial surface of each crown using a trephine mill ( Moraes, São José dos Campos, SP, Brazil) and polishing paper (Extec, Essington, PA, USA). Fluorescence Analysis Fluorescence measurements were performed with the RF5301 PC (Shimadzu Corp., Kyoto, Japan) spectrofluorophotometer with excitation wavelength at 365 nm.
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Freshly extracted nondamaged and intact bovine incisors were stored in a % thymol solution and refrigerated at 4°C, until required. Cylindrical enamel samples (3 mm in diameter and 1 mm height) were prepared from the labial surface of the tooth using a trephine mill (Dentoflex, São Paulo, SP, Brazil).
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For that, thirty extracted, nondamaged bovine intact incisors were stored in % thymol solution at room temperature until required. From each crown two enameldentine specimens, 3 mm in diameter and mm in height, were prepared from the labial surface with a trephine mill (Dentoflex, São Paulo, Brazil).
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Thirty extracted, nondamaged, intact bovine incisors were stored in % thymol solution at room temperature until required. From each crown, two enameldentin specimens, 3 mm in diameter and thick, were prepared from the labial surface using a trephine mill (Dentoflex, São Paulo, Brazil).
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trephine mill (Dentoflex, S˜ao Paulo, SP, Brazil). The enamel surface was ground flat and polished with watercooled silicon carbide (SiC) paper discs (1200, 2500, and 4000grit; FepaP, Panambra, S˜ao Paulo, SP, Brazil), thereby removing approximately 200µm of the outermost layer as verified with a micrometer (Micromar 40EXL, MahrGoettingen,
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